Sergio Garnica Galvez

7 Questions 7 Answers 0 Followers

Questions related from Sergio Garnica Galvez

RossetteSep STEMCELL TECHNOLOGIES CD4+ isolation kit from whole blood, anyone used it?

Hi every one, I would like to know if someone have used the RossetteSep kit from StemCell Technologies for CD4...

18 February 2022 7,835 4 View

Which is the best protocol for Alcian Blue staining and semi-quantification in bidimensional cultures?

Hello I am performing the chondrogenic differentiation of adipose-derived stem cells in bidimensional cultures. I would like to ask which one is the best protocol for the staining and the...

07 February 2018 966 6 View

How to avoid Mycoplasma contamination in horse samples?

Hi all, I am working with equine-adipose derived stem cells. After culturing the primary culture of the stroma and performing the first passage in our quarantine unit, I made the corresponding...

11 April 2017 1,349 2 View

Transport of fat tissues, ADSCs isolation?

Hello everybody, Two days ago I performed an isolation of adipose-derived stem cells. I think almost sure I did everything properly. But I observe the cells after more than 24 hours (let's say 36...

08 March 2017 5,824 2 View

Collagenase fat digestion?

Looking at the DataSheet of the collagenase for fat digestion in order to extract adipose-derived stem cells. It is said to dilute 1-2 mg of collagenase in TESCA buffer (ontaining 50 mM TES, 0.36...

27 February 2017 3,340 4 View

Which is the difference between EDTA and Sodium Azide for FACS analysis?

I saw for flow cytometry analysis, besides BSA 0.5-1%, the presence of EDTA at 2-3 mM or sodium azide 0.1% in the buffer. I thought to use as buffer 1% BSA and 3 mM of EDTA in PBS. But I do not...

09 February 2017 4,763 3 View

High-through put flow cytometry?

Hi everybody, I would like to perform a high through put flow cytometry analysis. I am new in this field and I would like to be sure before starting. I want to characterize several kind of cells...

06 February 2017 5,554 5 View