Sergey Bombin

5 Questions 7 Answers 0 Followers

Questions related from Sergey Bombin

How could I estimate allelic imbalance and/or genetic imprinting if I do not have separate paternal and maternal transcriptoms but only combined one?

Hello, I am trying to estimate allelic imbalance in C.remanei. Most of the methods that I found require to have separate maternal and paternal transcriptoms to estimate allilic imbalance based on...

01 April 2016 5,964 0 View

What formula could I use to calculate H2O2 based on absorbance?

I am trying to calculate H2O2 in plant tissue based on this protocol: Fresh leaves (0.5 g) were homogenized using acold mortar and pestle in precooled acetone (5 ml) and thehomogenate was...

06 October 2015 1,164 4 View

How could I quantitatively measure reactive oxygen species in plants?

I have only spectrophotometer, qPCR, microscope, some other basic stuff like centrifuge and a bunch of chemicals.

09 February 2015 6,002 9 View

Do you know the protocol for the infection of Arabidopsis plants with powdery mildew?

Hello, I need to infect my plants with powdery mildew to check if they become more susceptible to the infection. Currently in my lab, we just drop spores and try to do it as evenly as possible....

03 December 2014 7,311 4 View

Do anyone know any protocols for isolating and observing abnormalities in the photosystem?

I need to isolate and observe if something is wrong with the photosystems of my plants. If you know of any protocols for testing the photosystems please share with me.Thank you

03 November 2014 2,765 7 View