Senthil Kumar Thangaraj

4 Questions 12 Answers 0 Followers

Questions related from Senthil Kumar Thangaraj

How can I avoid adduct formation for high molecular weight protein using ESI-MS?

I am working on 260kDa iron-sulfur cluster containing protein using FTICR (ESI source) MS. We found the tetramer formation, but there is a strong adduct formation, not clear what kind of adduct it...

28 October 2016 271 4 View

Does anyone help me to find out the unknown Kd from a known Kd in a competitive titration experiment using mass spectrometry?

I am working on Protein-ligand interaction using mass spectrometry. We have found the Kd of protein-ligand complex by Titration experiment. Now we are doing a competitive titration to find the...

09 June 2016 9,454 3 View

How can we estimate dissociation constant (Kd) of high affinity protein using Native mass spectrometry?

I am working on Native mass spectrometry (FT-ICR, ESI source) to estimate protein ligand interactions. My protein that binds to the ligand with a sub-Nano molar dissociation constant. i am using...

13 April 2016 2,697 8 View

How do I calculate a dissociation constant using MATLAB?

Hello, I am working on Protein-ligand interaction. For that I would to like to calculate the dissociation constant. I have tried to do it using Excel but it doesn't work good. Then i found that...

17 April 2015 2,970 5 View