Senthamizh Gopal

3 Questions 3 Answers 0 Followers

Questions related from Senthamizh Gopal

Do slow growth umbilical cord tissue derived exosomes also have slower effect on cancer cell lines when treated?

We have been doing explant culture, we tend to see slow growth in cells despite of the correct media change, and other factors.its Day 25 and we still see 20-30 %confluent only in P0. Collecting...

21 June 2025 4,969 2 View

Why am I observing a layer formation while culturing U87 cell line?

They are cultured with DMEM supplemented with 10% FBS. What could be the reason for the layer formation? However, a persistent layer was observed forming on top of the culture the very next day,...

15 April 2025 2,279 2 View

How to quantify preotein concetration of EVs by bradford?

I have isolated extracellular vesicles (EVs) using PEG-based precipitation and removed PEG using a 50 kDa filtration step. I now want to quantify the protein concentration of the isolated EVs....

08 April 2025 4,336 3 View