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I had plated 10% FBS media to check for contamination and observed floating tree-like structure when checking the flask 15 days later. I had checked this flask daily initially and observed no...
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I am growing MEFs in a T75 flask in 15 ml high glucose and 10% FBS media each time. I started off with 2 million cells and they are reaching 70-80% confluency at the expected rate (within 2-3...
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ELISA seems like an easier method than Western BLot, but most papers that involve detection of p38 phosphorylation or P38 MAPK activity involve the Western Blot technique. Does anyone know of why...
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