Selami Demirci

7 Questions 13 Answers 0 Followers

Questions related from Selami Demirci

What is the best way to analyze HDR/INDEL frequencies for targeted deep sequencing data?

Hi everyone, We use the synthego ICE or TIDE online platforms for Sanger sequencing analysis for our CRISPR-edited cells with no problem. We also do amplicon deep sequencing (more than 50k read...

21 August 2022 8,259 0 View

Is there a way to grow human hematopoietic cells from transplanted NSG-mice peripheral blood in culture?

Is there a specific media that allow expansion of only human (not mouse) hematopoietic cells from transplanted NSG-mice peripheral blood in culture? As human cell number in mouse blood is very...

02 July 2021 3,150 5 View

Why are Sanger Sequencing reads interrupted in some edited clones?

I have a Crispr/Cas9 edited bulk population and I can briefly check the indel ratios in the bulk population using Sanger sequencing. After cloning single cells, I wanted to check the indel types...

19 February 2021 8,526 6 View

What would be a possible explanations for toxicity at low doses of a compound while there is not any cytotoxicity at higher doses?

I am conducting an in vitro cell proliferation assay. The results of repeated experiments revealed that there is 20% cell proliferation inhibition at the lowest doses, an average rate of %30...

16 March 2015 6,713 13 View

Any suggestions on wound dressing usage in animals?

Has anyone experience with wound dressing studies conducted with rats? Is there any established protocol? The first thing we wonder how we manage to keep the dressing stable on the dorsal side of...

25 February 2015 5,302 9 View

What are the best PI3K/Akt Signaling pathway antibodies for Western Blot?

I want to study PI3K/Akt Signaling pathway in human and mouse samples using Western Blot. Does anyone know antibodies for Akt signaling pathway intermediates which works great in Western Blot,...

24 October 2014 4,851 6 View

Is continuous puromycin treatment during the experiment necessary for gene therapy studies?

I have generated lentiviral mediated gene overexpression in fibroblast cells. I treated cells with puromycin (10 ug/ml) for 28 days and observe GFP (expressed as a reporter gene along with...

08 July 2014 1,254 4 View