Selami Demirci

4 Questions 8 Answers 0 Followers

Questions related from Selami Demirci

Is there a way to grow human hematopoietic cells from transplanted NSG-mice peripheral blood in culture?

Is there a specific media that allow expansion of only human (not mouse) hematopoietic cells from transplanted NSG-mice peripheral blood in culture? As human cell number in mouse blood is very...

02 July 2021 3,085 5 View

Why are Sanger Sequencing reads interrupted in some edited clones?

I have a Crispr/Cas9 edited bulk population and I can briefly check the indel ratios in the bulk population using Sanger sequencing. After cloning single cells, I wanted to check the indel types...

19 February 2021 8,368 6 View

What are the best PI3K/Akt Signaling pathway antibodies for Western Blot?

I want to study PI3K/Akt Signaling pathway in human and mouse samples using Western Blot. Does anyone know antibodies for Akt signaling pathway intermediates which works great in Western Blot,...

24 October 2014 4,772 6 View

Is continuous puromycin treatment during the experiment necessary for gene therapy studies?

I have generated lentiviral mediated gene overexpression in fibroblast cells. I treated cells with puromycin (10 ug/ml) for 28 days and observe GFP (expressed as a reporter gene along with...

08 July 2014 1,151 4 View