Sehrish Javaid

3 Questions 2 Answers 0 Followers

Questions related from Sehrish Javaid

Is it better to pool siRNA?

I am trying to knockdown two genes in my cells. i have optimized the cell number and concentration of the transfection reagent, but the western blots did not show a very effective reduction in...

03 March 2016 8,243 8 View

Is it normal for cells to grow in sheets in soft agar?

I am doing soft agar growth assays for the first time and my lab has not performed these assays extensively before either. I prepared my agar bases with 0.6% agar and suspended equal amount of...

01 January 2016 5,371 5 View

Has anyone experienced transfection failure for OKF cells?

I recently joined a lab and was handed a protocol and cells for transfection using fugene 6. I prepared the plasmid DNA and the GFP signal was good the first time. However ever since then, I have...

12 December 2015 3,381 3 View