Sedigheh Ranjbar

4 Questions 1 Answers 0 Followers

Questions related from Sedigheh Ranjbar

How can I increase the yield of separated IELs from mouse's colon?

I tried 3 times shake in HBSS and then used 30% percoll and gradient centrifuge with 40% and 70% percoll. But I need to increase the yield of IELs. Does anyone apply a different protocol?

13 September 2023 5,217 0 View

Antimicrobial peptides (AMPs) increase levels of pro-inflammatory cytokines through what mechanism?

In IBD , AMPs how or through what mechanism increase expression of inflammatory cytokines? Could someone please give me some advice on this?

17 July 2023 7,193 2 View

What is the reason for Ct difference greater than 0.5 between duplicate samples in PCR ?

Hello, I did PCR for mouse genotyping; the Ct difference for around half of my duplicate samples was greater than 0.5. I cannot find what the problem is. If someone could assist me with this...

25 May 2023 1,754 3 View

Mechanism of Anti microbial peptides (AMPs)

If anyone can speculate as to how AMPs promote inflammation in colitis or how AMPs raise the amount of inflammatory cytokines in colitis, I would appreciate it.

01 January 1970 9,174 1 View