Sd Micro

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Questions related from Sd Micro

How to remove protein from extracted bacterial DNA?

I tried to extract genomic DNA from gram positive bacteria using magnetic bead protocol. The recovered DNA I found was low and protein concentration was very high (although I used proteinase K...

19 May 2022 806 1 View

What can I do if I get unexpected plasmid after sequencing of bacterial DNA? how can I characterise the unknown plasmid?

After sequencing a bacteria Streptococcus iniae I have got a chromosomal DNA and a extra plasmid DNA. I have not found any evidence of Streptococcus iniae having plasmid!! is it possible to...

03 July 2020 6,445 8 View

Plasmid annotation tools

I have found plasmid sequence from bacterial whole genome sequencing. is there any tools for plasmid annotation?

01 January 1970 3,128 1 View