Scott Shaw

3 Questions 2 Answers 0 Followers

Questions related from Scott Shaw

What are good antibodies for avb3 and/or avb5 integrins which are not an agonists/antagonists?

I want to do ICC and measure the internalization of RGD-receptor integrins triggered by a RGD compound we're working on. I don't want the antibody to either block the RGD site or to trigger...

08 August 2016 741 0 View

What is the practical difference between collecting images using a 60x objective with 1.5x intermediate magnification versus using a 90x objective?

For reference, I'm using a Nikon T-2000U microscopy with a Hamamatsu Orca-Flash 4.0 CMOS camera. I'm capturing brightfield and epifluorescence images of cancer cells.

05 May 2015 3,692 4 View

Does anyone have experience with positive control for Endosome Escape?

Can anyone suggest an agent I could use as a positive control for endosome escape? I need something to which to compare my attempts at triggering endosome escape with photothermal heating. I'm...

10 October 2014 7,946 2 View