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Can I simply switch a construct-GFP from one plasmid (pEGFP-N1) to another (pCDNA) and would it cause any significant issues? There are no issues regarding restriction digest etc., and plasmid...
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Has anyone used NLS plasmids that work well for human cell transfections? Has anyone used the Cherry/ GFP ones, and do they affect protein function once in the nucleus (are non-tagged ones...
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I'm trying to figure out why certain nuclei in a population (in culture) might not be staining with DAPi. I am treating the cells with Leptomycin B to stop nuclear export of proteins in order to...
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