4 Questions 2 Answers 0 Followers
Questions related from Scott Yim
Hi everyone, i recently did a qPCR to check the best annealing temperature for my primers. So i ran two of my primers on annealing temperature 59C with a melting curve. The melting curve shows...
18 November 2020 2,099 5 View
Hi everyone, I just recently did some RNA extraction for my insects sample for RNA-seq. I have checked both the concentration (average 450ng/uL) and 260/280( ~2.0). I also done a agarose gel run...
22 October 2020 1,859 3 View
Hi all i am looking to design primer for the wingless gene in pea aphid (wnt-1) protein to look at the methylation status of the gene. Since i dont know where the methylation point is in the gene...
28 February 2019 6,219 11 View
I need to known a DNA sequence for a gene in the plant(V.faba) in order to design primer for PCR for DNA methylation purpose analysis.However I only had the mRNA seq and AA seq for the gene in...
19 October 2018 3,651 4 View
