Scott Mondrala

4 Questions 3 Answers 0 Followers

Questions related from Scott Mondrala

Detecting free cysteine on proteins?

Does anyone know of a method to detect the number of free cysteines on proteins? I would like to assess the binding of quinones to cysteines on an enzyme.

18 November 2023 7,676 2 View

Using raffinose to derepress the Gal promoter, does the media need glycerol as a carbon source?

I am overexpressing and purifying and enzyme from yeast. I know that I can use media with glucose for the starter culture and switch to raffinose to derepress the Gal promote and then add the...

04 January 2022 8,081 0 View

What is a good source of myeloperoxidase?

I need a fair amount of myeloperoxidase for my studies. I have previously purified from HL60 cells but get low yield at high cost. Is there another good source except from whole blood lymphocytes?

04 January 2022 7,989 3 View

Can HEPES be replaced with Tris when purifying protein with a intron-CBD tag?

Traditional purification of my protein is performed with Tris-Cl. All of the protocols I’ve found for purifying with the intern-CBD tag uses HEPES. Are they interchangeable for this purpose?

12 November 2021 8,798 5 View