Scott Russell Gregory

2 Questions 8 Answers 0 Followers

Questions related from Scott Russell Gregory

Loss of co-expression of GFP/GOI when transducing T cells with lentivirus?

Hi all, I am transducing murine CD8+ T cells (OT-Is) with concentrated lentivirus at various MOIs. (We calculated the MOIs by using a lentivirus quantification kit). I'm transducing the activated...

05 January 2022 1,772 6 View

Lentivirus concentration too high to transduce HEK293 cells?

Hi everyone, I am trying to transduce my HEK293 cells with lentivirus containing a GFP construct. Initially, I was getting a low GFP readout when assessing through FACS, so therefore we...

07 January 2021 2,140 15 View