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Hello, I am expressing an integral membrane protein (MW 51kDa) in Hi5 insect cells. I initially purified the protein by Nickel-His, concentrated it and subjected it to Size Exclusion...
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Hello, I am trying to crystallize a 16kDa protein. I was unable to express the protein in mammalian system but was successful in expressing it in BL21 (DE3) cells with the addition of a GST tag....
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Hello, I am trying to purify a GST tagged protein of 16kDa (Total MW with GST is 43KDa). I used affinity chromatography with GST beads to initially purify my protein and it yielded a fairly...
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I have a diffraction data set from a pseudo merohedrally twinned protein crystal and have no idea how to solve it. This crystal is very difficult to (re)produce and generally diffracts poorly. I...
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