Saurabh Tiwari

5 Questions 9 Answers 0 Followers

Questions related from Saurabh Tiwari

Mass-spectrometry vs. Western blotting?

Hi all, We validated an increase in our target protein concentration (after treatment) through Western blotting. This protein is moderately abundant in bovine sperm. However, we did not see this...

30 May 2023 4,615 6 View

Storage of primary antibody for Western Blotting?

Hi all, In our lab, we use Anti-Phosphotyrosine Antibody, clone 4G10® (Catalog no. 05-321, Millipore Sigma) to detect tyrosine-phosphorylated proteins through western blotting. I dilute the...

30 November 2022 4,815 5 View

Designing primers for mitochondrial transcripts?

Hi everyone, Please correct me if the information mentioned here is incorrect. In the animal mitochondrial genome, there are no introns coding sequences. I was wondering whether the primers...

07 June 2022 5,039 4 View

Does all protein get transfer from gel to the membrane in Western blotting?

Hi all, I am transferring the proteins (extracted from sperm in Laemmli buffer) to a 0.22µm nitrocellulose membrane at 100V for 60 min (wet-transfer). However, Coomassie blue gel staining after...

02 May 2022 2,374 3 View

Does the cholesterol content in plasma membrane affects the efficiency of cell lysis during initial process of RNA extraction?

Whether the pH of lysis solutions (while RNA extraction) need to be modified as per the cholesterol content in the cell's plasma membrane?

17 January 2022 4,325 3 View