Saumyadip Sarkar

6 Questions 13 Answers 0 Followers

Questions related from Saumyadip Sarkar

How to resolve this protein stability which gets aggregated?

I am using a bacterial protein and its get aggregated. I get a clear band of appropriate size in SDS PAGE but unfortunately it forms smear pattern in NATIVE PAGE. Also i try to check in TEM and...

11 November 2019 6,981 11 View

Does western blotting allow you to measure cytokine levels in plasma?

I want to measure the expression levels of a particular cytokine level in plasma with western blotting. I have primary antibody from ELISA. Does it require any purification? Please clarify my doubt.

12 December 2014 7,575 7 View

Is there any analogous protein for TGFB1 to detect in plasma through ELISA?

I dont have kit but rather have the antibodies and OPD to detect my samples. But problem does arise based on the standard samples. Any possible outcome apart from kit based assay. Yes making...

12 December 2014 6,231 4 View

Serum or Plasma is the best way to detect cytokines via ELISA?

Most of the paper suggests to do ELISA from Serum. But in our lab we collect plasma. Is there any difference to be found while doing ELISA with Serum and Plasma? NB: Blood was collected via EDTA...

11 November 2014 8,797 35 View

Any one know of Any RNA isolation kit to isolate RNA from pus cells and is RNeasy Mini Kit is favorable?

I want to isolate RNA from pus cells from middle ear of Otitis Media patients. Is RNeasy Mini Kit provided by Qiagen is favorable?

07 July 2014 8,899 6 View

If I need to choose between Affymetrix and Illumina DNA microarray chipset then which one should I choose?

It is a new assay for me in multiple SNP analysis with wide case and control range.

06 June 2014 2,775 2 View