Saumya Raizada

2 Questions 1 Answers 0 Followers

Questions related from Saumya Raizada

I have been trying to amplify a gene, but not able to do so. I have tried gradient at 45 50 55 60 and 65 but no use and even changed the RT.plz help?

the primer sequences are as: F-1 CTCTGTTCTCTCTTTCTCTCCTATC R-1 CACTTGACGGTATATTATGAGCTTG F0 GGATCCAAATGGCGTCCATC R0 GGATCCTTAAGTTCTGGTTCGAA

14 September 2019 9,199 0 View

I have cloned mapk3 in BL21 but I am not able get inducible expression of my protein kindly help?

I have cloned MAPK3 in BL21 cells. I have already tried protein induction at 37, 28 and 22 degree Celsius with IPTG conc ranging from 0.4mM to 2 M. But I got no protein induction. I have also...

17 July 2018 4,060 3 View