Saumya Prasad

4 Questions 1 Answers 0 Followers

Questions related from Saumya Prasad

Is it necessary to purify the PCR product before digestion?

I am trying to clone two genes in pET 28a vector using BamHI and XhoI sites and BamHI and EcoRI sites respectively. I am purifying the PCR product before digestion using Qiagen kit, but in the...

07 May 2018 8,247 8 View

What is the molar extinction coefficient of Tryptophan at 295 nm?

The extinction coefficient of Trp at 280 nm is around 5600 M-1cm-1. I wish to know its extinction coefficient at 295 nm.

22 November 2017 1,179 1 View

How can I calculate the center of mass for the NZ atoms of the lysine residues in my protein using VMD?

Can anyone please suggest me a tcl script for the same. I have tried using a script but its returning the same values for the center of mass for all the residues.

25 November 2014 3,942 1 View

I need to calculate the center of mass for the NZ atoms of the lysine residues in my protein. How can I do it using VMD??

Can anyone please suggest a tcl script for the same? I tried using a tcl script but its returning the same x,y,z values for all the lysine residues. The selection I used was "resname LYS and name...

25 November 2014 8,758 3 View