Saugat Pokhrel

3 Questions 6 Answers 0 Followers

Questions related from Saugat Pokhrel

What could be the possible critical reasons for failure of transient gene expression in onion epidermal peels using PDS 1000He device?

Hi, I have worked with onion epidermal cell transformation for a long time now. Did some successful transformations with high efficiency towards the end of last year and beginning of this year...

20 October 2016 9,512 7 View

Can anyone tell me how does the RNA from Arabidopsis leaves look like in a gel?

I have been working on extraction of mRNA of a gene of my interest in Arabidopsis. However, I am confused with what to expect in the gel after i run Total RNA (meaning mRNA, tRNA, rRNA) in a gel....

27 October 2015 7,655 3 View

Does GelRed modifies the migration rate of the DNA?

I did a preparative PCR for my cloning and run in a 1 % agarose gel, I see that the size of the amplicon is slightly around 80-100 bp less than i expected. there are no other bands. so its clear...

20 October 2015 780 5 View