What is a standard protocol for chicken embryonic myoblast culture (CEM)? How can I know that those are myoblasts or fibroblasts?

I was using 10th day embryo, DMEM 450 ml, 10% FBS 50 ml, streptomycin 5 ml, tripsin for CEM culture. Three times I failed due to contamination. Finally, I got good cell growth in all the six well...

26 March 2015 3,015 4 View