Satarupa Deb Sinha

4 Questions 6 Answers 0 Followers

Questions related from Satarupa Deb Sinha

How to predict tmRNA secondary structure?

Can anyone tell how can I predict the secondary structure of tmRNA of a specific species of bacteria(Leptospira interrogans) ?

19 April 2025 5,127 1 View

Having trouble in designing primers for a mutation?

5'...

17 February 2025 6,643 3 View

Why am I unable to transform my plasmid in BL21 cells and how can I express the protein?

I have my gene of interest in the plasmid pQE30. My gene of interest is AlaRS. I want to express the protein AlaRS . I obtained this plasmid from Addgene which says the plasmid grows in E. coli...

04 December 2024 4,251 6 View

Why my site-directed mutagenesis did not work?

I used the Q5® Site-Directed Mutagenesis Kit (NEB) to perform Site-directed mutagenesis of my plasmid(2886 bp) with an insert of 348 bp and Ampicillin resistant. I followed the protocol of NEB for...

18 November 2024 8,907 7 View