Satadru K Lahiri

3 Questions 7 Answers 0 Followers

Questions related from Satadru K Lahiri

CDK5 knockout cell line?

Can anyone suggest me where can I get CDK5 knockout cell line (preferably MEF)?

10 October 2013 5,460 0 View

Can anyone suggest an array to identify the kinase responsible for phosphorylation?

I found a putative serine site on a protein that is phosphorylated and shows 2 bands. When I treat it with CIP, it shows one band and the serine mutant protein also shows 1 band. So, we found the...

01 January 2013 236 19 View

Tagged protein expression

I cloned an ABC protein ORF of 4kb size in HA vector of 4.7 kb. I also sequenced to confirmed the construct, but when I transfect into 293 cells for overexpression, I always get a band higher than...

01 January 2013 8,033 17 View