Sasha Nealand

9 Questions 17 Answers 0 Followers

Questions related from Sasha Nealand

M. tuberculosis is very slow growing, can I still plate it for a disk diffusion assay as I did on the fast growing Mycobacteria sp I used to screen?

I used a non-pathogenic fast growing Mycobacteria to screen my natural product compounds for activity. Now I want to test the active compounds in a disk diffusion assay against pathogenic...

02 February 2020 7,998 3 View

What is a good nutrient agar for plating Mycobacteria tuberculosis for a disk diffusion test and why can't Mueller Hinton agar be used?

After I did some disc diffusion assays with a fast growing Mycobacteria I now want to do this using M. tuberculosis but I read that you cannot use Mueller Hinton agar with this. Does anyone know...

12 December 2019 8,002 3 View

Percentage of collection of solvent composition recovered from rotovap?

if I evaporate a mixture of hexane:ethyl acetate in a rotary evaporator under vacuum, will the solvent collected from the condenser have approximately the same ratio of hexane:ethyl acetate? bp...

08 August 2019 6,250 2 View

Is there any problem with using 0.1%TFA in HPLC mobile phase if the purpose is isolation, structure elucidation by NMR and bioassay?

07 July 2019 5,915 3 View

Is it possible to use hexane:ethyl acetate mobile phase on a reverse phase column?

I am reading a journal paper in which separation of a plant extract was done on a C18 HPLC column using normal phase solvents of hexane:ethyl acetate, is this possible?

07 July 2018 8,244 14 View

What is a good test mix for normal phase HPLC in Hexane:Ethyl Acetate?

What is a good test compound for normal phase HPLC in Hexane:Ethyl Acetate? I'm looking for a good test mix to make from common things in the lab for this to use on a silica column. I never used...

07 July 2018 3,814 6 View

When making a plant extraction what do the terms: work up? decolorization? acid and neutral fractions?

What does it mean in plant extraction when there is an acid fraction and a neutral fraction? How are these fractions obtained? Also when a journal article says The acid fraction was separated...

07 July 2018 7,647 6 View

How to dissolve insoluble sample in methanol or acetonitrile for LC/MS analysis?

I separated a crude plant extract into fractions on a silica column using ethyl acetate and hexane, now I want to look at the fractions using LC/MS. The problem is that our instrument room only...

07 July 2018 1,927 6 View

Plant extraction into Acetone?

Hello, what do you think of extraction of 1kg crude plant material directly into acetone? I wish to get out an alkaloid with a fatty tail, I wanted to use dichloromethane but we dont have this...

01 January 1970 7,925 16 View