Sarmad Al-Marsoummi

6 Questions 4 Answers 0 Followers

Questions related from Sarmad Al-Marsoummi

Proteasome Inhibitor that blocks Caspase-like activity?

Hi, Any suggestion for proteasomal inhibitor that blocks caspase-like activity of the proteasome in mammalian cells? MG132 and Bortezomib both acts on ChT-like acitvity! Thanx

07 November 2018 7,193 1 View

Time of MDA-MB-231 cell starvation in serum free media?

Hi, How long I need to starve MDA-MB-231 cells in serum free media to synchronize them before I infect them with my protein of interest using Adenovirus.. I'm aiming at conducting cell cycle...

23 December 2017 3,063 3 View

Bioluminescence for tumor metastasis?

Hello, What are the limitations and pitfalls of using luceiferase expressing cancer cells injected in Xenographt mice for studying the metastasis? Thank you

19 November 2017 7,457 2 View

STAT3 alpha isofrom siRNA

Hi Anybody was able to use siRNA to block STAT3 alpha specifically? Thanx

17 July 2017 2,011 1 View

What is the acceptable Passage number of MDA-MB-231cells?

Hi, I'm wondering what passage number is acceptable for MDA-MB-231 cells, Some labs kill these cells when they are 20-25 passages. Others Suggest it should be below 60 passages Thanks

05 July 2017 8,224 3 View

Hi I want to synchronize my MDA-Mb-231 cells and infect them with Adenovirus with protein of interest any suggested protocol?

I used MDa-MB-231 cells, interested in protein that I want to overexpress by using Adenovirus I want to synchronize the cells to do cell cycle analysis Any Suggested steps ? Thanx

13 June 2017 9,494 1 View