Sarfarazhussain Farooqui

3 Questions 7 Answers 0 Followers

Questions related from Sarfarazhussain Farooqui

Is the mitochondrial pellet always yellowish brown after mitoisolation, can it be pale whitish?

I did mitoisolation in zebrafish in the MB buffer of following composition: Filter-sterilize and store at 4°C for a maximum of 1 month. Consists of 210 mM mannitol, 70 mM sucrose, 1 mM EDTA, 10 mM...

08 November 2017 1,628 2 View

How to confirm if the pellet isolated from mitoisolation protocol is really mitochondria?

I am doing mitochondrial swelling assay using potassium acetate buffer. Rather than swelling, it shows shrinking phenotype raising doubts on purity of mitochondria. Is there a way to check it's...

06 November 2017 3,436 4 View

Do mitochondria from different species have completely similar ion exchangers?

I am trying to optimize swelling assay for isolated mitochondria from the zebrafish embryos. How much is it feasible that the assay used for yeast mitochondria isolation will work with zebrafish...

06 November 2017 1,204 1 View