Sarah Hemming

3 Questions 11 Answers 0 Followers

Questions related from Sarah Hemming

Can you freeze the secreted Nanoluc luciferase culture media for later luciferase activity analysis?

Is it ok and possible to remove the supernatant (cell media) containg secreted luciferase and freeze in a 96 well plate at -20 or -80 to thaw later for luciferase assay. I am using a secreted...

10 October 2016 4,739 4 View

Can we extract RNA from 95% ethanol fixed tissues?

Hi, I was wondering if it is possible to extract RNA from tissues which have been fixed in 95% ethanol and stored at RT. The RNA would be used in RT-PCR analysis. If anyone knows a protocol or...

03 March 2015 8,668 12 View

Can anyone recommend a ChIP grade JMJD3 antibody other than the current ab85392 abcam antibody?

Hi all, Has anyone been able to successfully use the ab85392 JMJD3 antibody for ChIP, if so what conditions did you use? I haven't been able to get this antibody to IP and obtain any bands, in...

11 November 2014 6,305 3 View