Sarah Sivilich

6 Questions 16 Answers 0 Followers

Questions related from Sarah Sivilich

Do you have any input for our new high volume tube line?

Micronic is proud to be an industry leading innovator, producer, and manufacturer of a comprehensive line of sample storage solutions. We are currently working on developing tubes with a larger...

14 February 2018 8,411 0 View

What are your reasons for preferring external or internal threaded tubes for sample storage?

Which is better for minimizing cross-contamination and why? Is an o-ring essential for you and why?

19 December 2017 4,979 0 View

Can you use neomycin PCR to distinguish between heterozygous and WT transgenic mice?

Hi friends! I've been working with knock-in heterozygous mice with ~10 point mutations in the Cald1 gene (homozygous lethal). After have trouble for a long time with genotyping, I outsourced...

11 March 2015 176 3 View

How can you make PCR specific to one allele?

Hi Friends, I have been having a nightmare genotyping these transgenic mice. What I have are heterozygous mice with a few knock-in point mutations. Originally, we used the same WT reverse primer...

24 November 2014 7,261 9 View

What is the best antibody to detect ubiquitination for my protein MKP-1?

I would like to test for ubiquitination in my samples, but don't want to end up with a smeared blot. Can anyone recommend a good antibody to use? Or does any company make anti-ubiquitin MKP-1...

09 May 2014 4,700 1 View

Can a stripped blot still be used for phosphoprotein detection? Thanks!

PVDF membrane - only stripped once.

16 January 2014 1,583 4 View