Sarah Sivilich

3 Questions 14 Answers 0 Followers

Questions related from Sarah Sivilich

How can you make PCR specific to one allele?

Hi Friends, I have been having a nightmare genotyping these transgenic mice. What I have are heterozygous mice with a few knock-in point mutations. Originally, we used the same WT reverse primer...

24 November 2014 7,202 9 View

What is the best antibody to detect ubiquitination for my protein MKP-1?

I would like to test for ubiquitination in my samples, but don't want to end up with a smeared blot. Can anyone recommend a good antibody to use? Or does any company make anti-ubiquitin MKP-1...

09 May 2014 4,646 1 View

Can a stripped blot still be used for phosphoprotein detection? Thanks!

PVDF membrane - only stripped once.

16 January 2014 1,520 4 View