Analysis of His phosphorylation with PhosTag gel?

Hi, I tried to analyze the phosphorylation status of my protein with the PhosTag acrylamide method. For this I lysed my HEK cells with RIPA buffer (50 mM HEPES-KOH, 150 mM NaCl, 1 mM EDTA, 1%...

23 October 2017 9,848 5 View