Sara Sabihi

3 Questions 5 Answers 0 Followers

Questions related from Sara Sabihi

Any advice on CFOS and GABA immunofluorescence in medial prefrontal cortex?

I'd like to fluorescently stain for activation of GABAergic neurons in the mPFC- but have a few questions. 1) When looking at the mPFC, would it be best to use antibodies for FOS and GABA, or FOS...

19 November 2015 282 5 View

Does anyone have experience with cannulating a brain region and then IHC staining for cFOS in this same region? Is it difficult due to tissue damage?

I would like to administer a drug centrally into a specific brain region and then look at activation of neurons in this same region as a result via IHC staining for cFOS. However, because there...

25 September 2014 2,074 5 View

Is ACTH peptide extraction from plasma required for ELISA?

I have performed a footshock stress protocol on rats and I am measuring ACTH from plasma. I have already extracted the plasma and it is frozen. I am preparing to run an ELISA on the samples using...

10 September 2014 2,412 2 View