Sara Parker

6 Questions 13 Answers 0 Followers

Questions related from Sara Parker

Sex-specific transcript for RNAseq?

Hi! I'm hoping to compare transcriptional differences between male and female samples (mouse, human) in RNAseq datasets of brain tissue that do not differentiate samples by sex. Does anyone have...

01 February 2019 6,536 3 View

How can I find out which proteins possess a specific domain or motif?

Hi! I want to generate a list of putative binding partners for my protein of interest by essentially BLASTing for the ligand which binds to EVH1 domains, FPxΦP. Is there a database where I could...

11 December 2018 8,903 2 View

Multisite Gateway Problem - Mosaic Expression?

Hello ResearchGate Community! I'm building polycistronic lentiviral vectors for the expression of multiple fluorescently-tagged proteins using Multisite Gateway, and I'm finding that I get mosaic...

03 November 2016 4,426 2 View

What equipment is required for FLIM-FRET?

Hi World, I'm having trouble finding a clear answer out there. We are trying to determine if FLIM-FRET can be performed using our widefield (or TIRF) microscope and an EMCCD camera. I generally...

26 August 2016 330 2 View

Uncaging glutamate (RuBi) on dissociated neuron cultures?

Does anyone have a good uncaging protocol for neurons in culture? I'm experiencing cell death after the addition of 30uM RuBi Glutamate to my DIV14 dissociated cortical neuron cultures. I am...

26 July 2016 435 2 View

How can I get IF to work on OCT-less mouse brain sections?

I am struggling with getting immunofluorescence working on my mouse brain slices. It seems that most antibodies, despite testing various concentrations, are not working out, so I was hoping for a...

27 February 2014 1,832 2 View