Sara Hernando

4 Questions 6 Answers 0 Followers

Questions related from Sara Hernando

How to densitometer angiogenesis in rat brain cortex using ImageJ?

I have performed an immunostainning in rat brain brain cortex in order to see new blood vessels formation. In order to cuantify it, I have densitometered the images following these steps in...

30 January 2019 3,428 0 View

Microglia primary cell culture purification. Are these good immunofluorescence results?

Hi everybody, I'm trying to obtain microglia purification from rat pupets from 1-3 days. After having the glia culture for two weeks, I tried to purify the microglia following this protocol...

19 December 2018 5,185 2 View

Does anyone have experience with anti-Nrf2 inmunohistochemistry protocol for rat paraformaldehyde fixed brain tissue?

Hi! I have purchased Nrf2 Abcam antibody (ab31163). I want to perform an inmunohistochemistry in paraformaldehyde fixed rat brain sections. I have not clear which protocol should I follow,...

11 December 2017 1,506 3 View

Can I take the results obtained from the Cylinder test in 6-OHDA rats to evaluate other tasks such anxiety as it was an open field test ?

Hi! I have perform cylinder test in 6-OHDA animal model of PD to test the neuroprotective effect of one drug. I have obtained good results measuring the number of impaired forelimb wall contacts....

17 November 2017 4,424 3 View