Sanyam Jain

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Questions related from Sanyam Jain

What is a safe dilution for drug stock solution (in DMSO) for injection into mice?

Hello folks! I wish to administer Doxorubicin to mice via the IP route (4 doses, with 3-day intervals). I wanted to know what would be a safe dilution of Doxorubicin (dissolved in DMSO) in PBS to...

05 August 2025 1,135 1 View

MSCs do not grow in CFU-F assay, what could be the reasons?

Hey folks! I am isolating MSCs from compact bone following this protocol- https://www.nature.com/articles/nprot.2009.238. Whenever I seed them for the CFU-F assay (density = 100 cells/cm2), cells...

29 July 2025 7,221 2 View