Santiago Ospina Areiza

4 Questions 1 Answers 0 Followers

Questions related from Santiago Ospina Areiza

¿Is "targets.txt" file always needed to analyze microarray data in R?

I´m trying to analyze the data from Illumina microarray using the Giovanni Coppola guide. I've also used some examples from the lumi package (bioconductor) and always use the targets file. The...

25 February 2020 4,099 3 View

Does M. bovis kill BMDC?

Hello, I'm trying to infect bone marrow dendritic cells with M. bovis at different M.O.I. Whats the recommended and maximum amount of time I should allow the infection before it kills the BMDC?...

31 May 2019 9,688 1 View

What is the maximum concentration of 2-mercaptoethanol for cell culture?

Hi, I am preparing media for cell culture (murine immune cells) and I wanted to know what is the maximum amount of 2-mercaptoethanol in a 500 mL media cell culture stock. Usually I would add 50...

29 May 2019 8,986 9 View

What is the relation between OD600 and concentration of Mycobacterium bovis Danish?

I'm growing BCG-D and I need it to be at OD600 of 0.6. What's the density equivalent to this OD? (number of bacteria/ml). Thank you.

27 April 2019 1,257 3 View