Can you help me to make the solution for TGase activity according to the method of Takagi et al (1986)?

The reaction mixture contained 1 mg/mL DMC, 15 mM MDC, 70 mMTris–Cl (pH 7.5), 5 mM CaCl2, 3 mM DTT and 100 mL of crude enzyme. Thereaction was performed at 37C for 10 min. EDTA was added to...

17 December 2016 7,600 2 View