Santha Ann Lim

3 Questions 3 Answers 0 Followers

Questions related from Santha Ann Lim

Anyone have the protocol for nalysis of Acridine Orange staining using confocal microscopy or microplate reader?

I am trying to replace fluorescence microscopy observation for the detection of acidic vascular orange with Acridine Orange staining. Anyone has experience in analyzing AO staining with confocal...

05 September 2019 406 1 View

Apart from western blot, what are the other tests that can be used to determine expression of autophagy-related proteins ?

I would like to know if there are other options that requires less cost.

10 July 2019 7,112 2 View

Preparation of Phosphate-buffered saline pH7.4 use Na2HPO4 or NaH2PO4?

I get confused between whether to use Na2HPO4 or NaH2PO4. As the are some protocol that uses Na2HPO4 and some that use NaH2PO4. I am using this PBS for cell culture and maintenance purpose. Need...

17 May 2019 447 4 View