Sanny David Pacheco Lumayno

2 Questions 10 Answers 0 Followers

Questions related from Sanny David Pacheco Lumayno

How do you increase DNA concentration of your PCR Product?

Good day Sir/Maám, I am using Fastgene PCR/ Gel Extraction Kit in order for me to purify DNA from PCR Product (I had a total of 50 ul solution). However, I am getting a low concentration 10 to 15...

29 January 2019 5,546 17 View

Is it necessary to design Primers from the start codon to stop codon (whole opening reading frame) for Reporter gene Assay?

Hello everyone! I am currently having problems with my Fw and Rv primers (including restriction enzymes) to subclone my target gene to pcDNA3.1. I have designed the Fw from the Start codon and Rv...

13 June 2018 1,694 9 View