Sanna Gudmundsson

6 Questions 12 Answers 0 Followers

Questions related from Sanna Gudmundsson

How can I calculate the likelihood of a rare variant segregating with a disease in a large pedigree?

Dear everyone, I would like to have statistics backing up a likely pathogenic rare variant that is segregating with the disease in a five-generation family. We have sequencing data from 17...

08 August 2018 7,186 0 View

Suggestions for Optimization of PCR for quantitative microsatellite readout?

Hi, Im doing a HUMARA assay to investigate X-chromosome inactivation. Right now the shorter allele has a great advantage in my PCR resulting in very different allele frequncies for my...

09 September 2016 525 4 View

What is the capacity of pEGFP-C1 and pUC19 DNA vector?

Hi all, Im searching to find some info about how large fragments can been cloned into pEGFP-C1 or pUC19 DNA vector. If you have done any cloning using this vectors, can you please state here how...

08 August 2016 3,736 13 View

Good vector for mRNA expression (large fragment!) in zebrafish?

Hi, I have been trying to clone an about 6kb large gene in to a vector that later is gonna be used for mRNA expression zebrafish. We have been using the pCS2+ vector as it was provided by colleges...

07 July 2016 1,458 6 View

Best kit for extracting long fragments of total RNA from HeLa cells?

Hi, I want to extract RNA form HeLa cells for PCR amplification of a 5.8kb large gene. Do you have any suggestions of what kit o use for the RNA extraction? Or would you go with trizol protocol?...

11 November 2015 753 4 View

Suggestions on cDNA kit for large fragments (>7b) and experience of Qiagen Long Range Kit for PCR?

Hi, I want to amplify a large gene

08 August 2015 3,928 4 View