Sanna Gudmundsson

6 Questions 16 Answers 0 Followers

Questions related from Sanna Gudmundsson

Method for freezing zebrafish embryos

Hi all, So I want to freeze zebrafish embryos to later (1 week) extract RNA with trizol + RNeasy kit an perform RNA-seq. Would you freeze the embryos in TRIzol or in RNAlater? Does to freezing...

19 June 2017 4,197 4 View

RNA extraction and poly A selection from 3dpf zebrafish

Hi all! :) I want to extract RNA from zebrafish embryos, 3dpf. My limitation will be the number of fish needed to gain enough material after (1) extracting total RNA (2) doing poly A...

15 June 2017 5,372 7 View

What is the capacity of pEGFP-C1 and pUC19 DNA vector?

Hi all, Im searching to find some info about how large fragments can been cloned into pEGFP-C1 or pUC19 DNA vector. If you have done any cloning using this vectors, can you please state here how...

17 August 2016 677 13 View

Good vector for mRNA expression (large fragment!) in zebrafish?

Hi, I have been trying to clone an about 6kb large gene in to a vector that later is gonna be used for mRNA expression zebrafish. We have been using the pCS2+ vector as it was provided by colleges...

06 July 2016 4,651 4 View

Large fragments >7kb with Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit?

Hi, Does anyone have experience of amplifying large fragments >7kb from cDNA obtained with Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit?BR Sanna

21 August 2015 1,097 5 View

Suggestions on cDNA kit for large fragments (>7b) and experience of Qiagen Long Range Kit for PCR?

Hi, I want to amplify a large gene

18 August 2015 3,765 4 View