Sanna Abbasi

3 Questions 5 Answers 0 Followers

Questions related from Sanna Abbasi

Are there 4 copies of Chromosome 17 and 22 in the HEK293 cells?

Recently, I looked up the HEK293 cell line on Wikipedia, and it states that in addition to 3 copies of Chromosome X, HEK293 cells contain 4 copies of both Chromosome 17 and 22. Is this true? The...

28 September 2017 8,968 8 View

Dark brown/yellow precipitate observed after speed vacuuming protein sample for LC-ESI-MS/MS?

Hi, does anyone have an idea what might be causing this dark brown (pale, clear yellow if I re-suspend) precipitate to form? Here is some background on my sample preparation: 1. Lyse HEK293 cells...

30 August 2017 9,032 7 View

Transformation of DH5-alpha cells with mutant gene yields wild-type from minipreps?

Hello, I just wanted to know if anyone has ever experienced anything like this, where transforming DH5-alpha cells with DNA results in the DNA itself being modified? I have checked the sequence of...

05 October 2016 2,473 3 View