Sanna Abbasi

2 Questions 3 Answers 0 Followers

Questions related from Sanna Abbasi

Dark brown/yellow precipitate observed after speed vacuuming protein sample for LC-ESI-MS/MS?

Hi, does anyone have an idea what might be causing this dark brown (pale, clear yellow if I re-suspend) precipitate to form? Here is some background on my sample preparation: 1. Lyse HEK293 cells...

30 August 2017 8,962 7 View

Transformation of DH5-alpha cells with mutant gene yields wild-type from minipreps?

Hello, I just wanted to know if anyone has ever experienced anything like this, where transforming DH5-alpha cells with DNA results in the DNA itself being modified? I have checked the sequence of...

05 October 2016 2,409 3 View