Sanjay Pratap Singh

4 Questions 17 Answers 0 Followers

Questions related from Sanjay Pratap Singh

Can anyone tell me Caco-2 cell line protocol by which it should be maintained in the lab lab during cell culturing?

It is the first time I am going to work on this cell-line.

03 March 2014 2,988 5 View

How do you measure directional Caco-2 permeability of test compounds in cultured Caco-2 monolayer for Aflatoxin B1?

The Caco-2 cell monolayer mimics the human intestinal epithelium. One of the functional differences between normal cells and Caco-2 cells is the lack of expression of the cytochrome P450 isozymes...

03 March 2014 1,849 0 View

Can anyone tell me the best solvent for AFB1 which minimises loss during storage?

Aflatoxin during storage in a solvent like methanol, acetonitrile, chloroform, etc., lose stability also these solvents are volatile, so concentration may change during storage. What proportion of...

03 March 2014 1,599 3 View

Do you know about the solubility and stability of Aflatoxin B1 in different solvent, temperature and light conditions?

Aflatoxin B1 is soluble in organic solvent like Chloroform, Benzene, Methanol, Acetonitrile etc. But after time it may loose quantity. Also temperature and light are degrading aflatoxin B1....

01 January 2014 7,320 7 View