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Questions related from Sanjana Skumar
I wanted to study structural /functional group changes of a compound at alkaline buffer (pH 12). But for buffers, salts and NaOH used will have hydrogen in them. So the solvent peak will mask all...
01 January 2020 1,771 5 View
The compound is isolated from a plant extract and there are two possible positional isomers. Their peaks are very close that sometimes it looks like a single peak with a small shoulder. I have...
02 February 2019 2,124 17 View
The sample (ethyl acetate plant extract (1mg/ml)) was prepared in methanol and the injection volume is 20ul. I have tried three different mobile phases: gradient 0-100% acetonitrile in water,...
11 November 2017 6,830 13 View
I have a mixture of compounds from which one is forming crystals. From fractional crystallization, I found that the impurities and the crystals dissolve in the same solvents. Also, the crystals...
08 August 2017 9,414 11 View
The sample is plant aqueous extract. After performing adsorption and desorption, an unknown compound was obtain and purified by re-crystallization. But, the NMR spectrum of the compound (in D2O)...
05 May 2017 2,875 5 View
The flavonoid of interest is polar in nature. The flavonoids were adsorbed onto acid pre-treated activated charcoal (packed in column) from ethyl acetate solution. I tried recovering with...
08 August 2016 6,415 9 View
I want to determine the concentration of a flavonoid molecule. The error due to weighing is quite high as the amount of sample is less. So, I thought of trying biochemical tests like Aluminium...
01 January 1970 7,863 3 View
