Sangeen Khan

4 Questions 4 Answers 0 Followers

Questions related from Sangeen Khan

Why after passaging the stable cells i can't get the same gene knockdown efficiency?

I used shRNA plasmid for the knockdown of gene. Firstly, package the virus, then transfect the target cells and then puromycin selection to get the stable cells. After selection of stable cells i...

28 March 2022 7,702 1 View

Can anyone help me what these black long thread like structures are?

I have grown 22Rv1 cells for virus transfection. After i added the virus the cells were clean but when i added puromycin for selection of stable cells then after 48 hours these black thread like...

28 December 2021 2,708 3 View

Can someone help me find out what are the black spots or black things between the cells?

These days in most of our cells we have these black small things in between the cells. when we see through ordinary microscope the cells looks fine and clear. But when we see through fluorescence...

15 October 2021 4,949 3 View

I am growing NIH3T3 cells, would highly appreciate if you can figure what is the problem in it?

I am growing NIH3T3 cells and i have tried so much but cant get 70-80% confluence. After 24 hours the medium gets turbid. Also there is a lipid like layer attached to the wall. if you can please...

24 June 2021 437 4 View