Sandra Martínez-Turiño

4 Questions 2 Answers 0 Followers

Questions related from Sandra Martínez-Turiño

Negative control recognition when using CTD110.6?

Hi there, After a good previous experience using the O-GlcNAc-Specific Antibody CTD110.6, WBs do not work anymore. In particular a negative control (previous used) and a new negative control...

16 September 2022 6,014 2 View

Synthetic antibody production company?

Hi there, We need an antibody for a plant protein recognition (100 kDa). No commercially versions are available. Antigen is not purified, so our own rabbit immunization is rule out at now. Could...

19 May 2022 4,417 5 View

To study protein degradation rate completely in vivo, there's some protocol to deliver cycloheximide by infiltrating leaves?

I´m trying to study degradation rate of a viral protein in Nicotiana benthamiana plants, but I would prefer do it absolutely in vivo (avoiding disc leaves for instance). After treatment protein...

02 September 2019 2,334 0 View

How do you improve the protein resolution in polyacrylamide gels?

I need to separate different forms of a protein around 36 kDa: non-glycosylated, mono-glycosylated, di-glycosylated, tri-glycosilated and tetra-glycosylated. My goal is to detect these proteins...

04 December 2013 9,323 13 View