Sandeep Vellanki

5 Questions 10 Answers 0 Followers

Questions related from Sandeep Vellanki

Which marker is good for measuring endothelial cell proliferation in brain and kidneys of mice?

I am planning on performing IF staining on mice brain sections. Should I opt for CD31/von Willebrand factor?

08 August 2018 3,653 7 View

Can RT-PCR be used to validate neutralization of a cytokine in cell culture?

For my experiment I will be treating HUVEC cell cultures with FGF-2 monoclonal nAb for 24 hrs. Since this cytokine works in an autocrine fashion, I was wondering if RT-PCR can be used to confirm...

12 December 2017 2,166 5 View

How to confirm neutralization of a cytokine by an antibody?

I am planning an experiment to determine if the expression of a certain cytokine (lets say IL-6) is dependent on the expression of another cytokine (lets say IL-11) in endothelial cells (in...

10 October 2017 3,570 4 View

How to measure hydrophobicity of drug in cell culture?

I have 3 different drugs and I want to test which one is more hydrophobic

06 June 2016 7,045 1 View

I want to separate cell debris from cell media collected from different wells in order to perform ELISA. What is recommended centrifugation or filter?

I want to separate cell debris from cell media collected from different wells in order to perform ELISA. What is recommended centrifugation or filtering? If centrifugation, what speed usually works?

08 August 2015 236 3 View