Sandeep Agrawal

3 Questions 5 Answers 0 Followers

Questions related from Sandeep Agrawal

What is the rough amount of antibodies per million cells to be used for staining intra-cellular proteins in a flow cytometry experiment?

population of interest is of osteoblasts

02 February 2016 1,198 3 View

Can the brain tissue be utilized for gene expression studies after death of the concerned subjects?

I was wondering about the viability of brain tissue after death. What I read is that brain tissue remains viable even 12-24 hours after death. But will the proteins in brain tissue and more...

06 June 2014 1,525 1 View

What should the primer concentration be for real time PCR?

I am using SYBR Green qPCR mastermix in Bio-Rad CFX-96 qPCR machine. For a 25 microlitre reaction of normal PCR, I use 0.75 miclolitres of 20 picomolar concentration, each of forward and reverse...

06 June 2014 1,309 5 View