Sanchita Mallick

3 Questions 3 Answers 0 Followers

Questions related from Sanchita Mallick

Why do I experience random death of cells after passaging?

Hello Everyone, I am culturing few different kinds of adherent cells. However, I sometimes experience cells dying in the flask after passaging them in one flask whereas same cells suspension...

08 February 2024 4,229 0 View

How to trypsinize IMR90 cells to get good viability?

I have a question regarding culturing fibroblast IMR90. I use DMEM+10%fbs+1% pen/strep for culturing them. I use 0.05% trypsin EDTA for trypsinization for 40 sec in the incubator and add DMEM +...

19 January 2024 906 1 View

IMR90 cell viability and immunoflorescence for alpha SMA staining?

I have a question regarding culturing fibroblast IMR90. I use DMEM+10%fbs+1% pen/strep for culturing them. I use 0.05% trypsin EDTA for trypsinization for 40 sec in the incubator and add DMEM +...

21 December 2023 4,312 0 View