Sana George

3 Questions 4 Answers 0 Followers

Questions related from Sana George

What could be the reason why Nocodazole arrested cells show up in G1 instead of G2/M?Does this mean Nocodazole failed to induce cellcycle arrest?

Hello, I have carried out cell cycle assay of HeLa cells using Nocodazole(40ng/ml) and performed FACS of the samples collected at different time points. Since I am new to this technique, I feel a...

03 August 2019 4,653 4 View

What issues could arise if the dilution of primary antibodies used for detecting GFP tagged protein by Immunofluorescence was 1:100 instead of 1:1000?

The prescribed dilution for the Anti GFP Antibody is 1:1000, however added 1:100 dilution instead. The two other antibodies are added at right dilutions and also the secondaries. I saw different...

27 June 2019 6,343 3 View

What is the optimum time required for treating transfected HeLa cells with Doxycycline to induce the expression of recombinant GFP tagged protein?

Hi all, I am going to perform immunofluorescence analysis of transfected HeLa cells to determine the localization of the protein of interest.The protein is tagged with GFP and I am using...

06 June 2019 2,257 2 View