Samuel Major

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Questions related from Samuel Major

What biomolecules are fluorescing red at band pass filters at 470/40, 525/50, 545/25, and 605/70?

I'm counting environmental samples stained with DAPI and counting the bacterial to algal ratios by counting the stained bacteria vs the autofluorescence of the chloroplasts. I'm using a Zeiss...

09 September 2017 9,276 0 View

What is the lowest concentration you have used to stain fixed bacterial cells with DAPI?

I diluted my stock DAPI, 5mg/mL (14.3 mM) in diWater, 1 to 10 in 1x PBS and it looks a bit like green curdled milk. I assume this is a strange reaction with the PBS salts. I used this...

03 March 2017 8,909 1 View

Will flowcytometry using a BD Accuri 6 FCM, be able to discriminate between SYBR-Green, Nile Red, and Chloroplast to count/ID bacteria and algae?

I am going to run a flow cytometry experiment on synthetic communities of microalgae and bacteria. I would like to use a nuclear stain to determine all the biological particles, and gate those to...

01 January 1970 4,924 2 View