Samuel Davis

3 Questions 33 Answers 0 Followers

Questions related from Samuel Davis

Improving TEV cleavage efficiency - does 50 mM biotin affect activity?

Hi, I'm expressing and purifying a membrane protein which at the C-terminus has a short linker followed by a TEV-mEmerald-TwinStrep tag. I can solubilise and purify the protein with...

02 January 2021 2,406 7 View

In-gel GFP fluorescence with SDS-PAGE protocol?

Can anyone give me some pointers on how to carry out an SDS-PAGE in-gel fluorescence assay with a uvGFP tagged membrane protein? From various protocols I've come across, I understand that I can...

09 September 2018 6,708 9 View

Problems removing RT- bands in RT-PCR - any suggestions?

Hi, At the moment I'm trying to acertain the endogenous gene expression of hP2Y receptor subtypes in the tsA201 cell line by reverse transcriptase PCR, however I keep having problems with bands...

29 June 2016 7,542 13 View